Journal: Cell Death Discovery

Article Title: New AKT-dependent mechanisms of anti-COVID-19 action of high-CBD Cannabis sativa extracts

doi: 10.1038/s41420-022-00876-y

Figure Lengend Snippet: A Western blot analysis of ACE2 and TMPRSS2 in WI-38 cells treated with the indicated most abundant compounds in extract #1, with the same concentration found in the extract; relative densitometry was measured using ImageJ. B Western blot analysis of ACE2 and TMPRSS2 in BJ-5ta cells treated with the indicated most abundant compounds in extract #7, with the same concentration found in the extract; relative densitometry was measured using ImageJ. C Western blot analysis of ACE2 and TMPRSS2 in WI-38 cells treated with either CBD or PTWT2.2 individually or in combination; relative densitometry was measured using ImageJ. D Western blot analysis of ACE2 and TMPRSS2 in WI-38 cells treated with either CBD or PTWT1.2 individually or in combination; relative densitometry was measured using ImageJ. PTWT represents terpene. * indicates p < 0.05; ** indicates p < 0.01.

Article Snippet: Human normal foreskin fibroblasts (BJ-5ta), purchased from American Type Culture Collection (ATCC, Manassas, USA), were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Western Blot, Concentration Assay

Journal: BMC Microbiology

Article Title: First human cell-based cultivation system for the syphilis spirochete Treponema pallidum

doi: 10.1186/s12866-026-04856-5

Figure Lengend Snippet: Human foreskin fibroblast cell lines support the growth of T. pallidum in vitro . A Human foreskin fibroblast cell lines (MoNa, HFF1, and HFFC) support the growth of T. pallidum in long-term, continuous, in vitro cultivation. While cultivation of strain SS14 was discontinued after 10 weeks, in vitro cultures of the DAL-1 strain have been ongoing for over one year. During this period, scheme of subcultures was optimized (up to 1:20). A booster passage (*), extending the standard 7-day subculture to a 14-day period with a cultivation medium exchange on day 7, was employed to enrich the treponemal culture during critical declines. In vitro cultivation was performed in triplicate (i.e., in three cultivation wells) and the mean values for each subculture are presented in graph. Source data from individual wells are shown in Supplementary Table . B Parallel cultivation for seven days ( n = 15) with defined DAL-1 inoculum (10 6 treponemes) validated the differences in growth support of individual foreskin cell lines. Treponemal growth on human foreskin fibroblast cells was slower compared to growth on rabbit epithelial Sf1Ep cells. Red bar, mean. C Human foreskin-based cultivation system supports the growth of various T. pallidum strains ( n = 8), from the Nichols-like as well as the SS14-like cluster. Note that human foreskin fibroblast cells were prepared as an equal mixture of three tested cell lines. Each T. pallidum strain was cultivated in a single in vitro well, representing a sole experimental replicate used for data acquisition

Article Snippet: Human foreskin fibroblasts HFF1 (SCRC-1041; ATCC) and HFFC (300715; Cytion) were purchased, while the third cell line (MoNa) was kindly provided by Dr. Vladimir Rotrekl (Masaryk University), and was originally obtained from the National Tissue Centre (Czech Republic).

Techniques: In Vitro

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 3. 2D-PAGE image from human fibroblast(control).

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques: Control

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 4. 2D-PAGE image from human fibroblast. Cell treated with AX. Extract.

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques:

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 2. Cell toxicity of fibroblast treated with AX extract.

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques:

Journal: AIDS Research and Human Retroviruses

Article Title: Purging Exhausted Virus-Specific CD8 T Cell Phenotypes by Somatic Cell Reprogramming

doi: 10.1089/aid.2017.0161

Figure Lengend Snippet: Establishment of B6/129OKSM mouse line. (A) Schematic illustration of B6/129OKSM mouse generation. We first established a DOX-reprogrammable fibroblast-like cell line originated from AinV15 mouse ESCs by transduction of STEM-CCA lentiviral vector encoding four DOX-inducible mouse reprogramming genes (Oct4, Klf4, Sox2, and c-Myc). Chimeric mice were generated by the injection of 8–12 established iPSCs into a B6 mouse blastocyst. The mice were bred with B6 mice to obtain F1 hybrids derived from 129P2/OlaHsd strain (agouti coat). F1 hybrids were then crossbred for >10 generations until we established a stable mouse line carrying a single copy of a reprogramming gene cassette introduced by STEM-CCA lentiviral vector homozygously and enabling induction of ESC-like colony formation from peripheral blood cells with high efficiency in the presence of DOX. DOX: doxycycline. Diff.: differentiation. (B) Fibroblasts were isolated from tail-tip biopsies of chimeric mice and exposed to 1 μg/ml of DOX for 2° iPSC induction. No 2° iPSC formation was seen with no DOX addition. (C) Expression of two pluripotent stem cell markers (Nanog and SSEA1) in 2° iPSCs. (D) Embryoid bodies generated from 2° iPSCs. iPSC, induced pluripotent stem cell.

Article Snippet: AinV15 mouse embryonic stem cells (ESCs) were purchased from American Type Culture Collection (ATCC SCRC-1029, Manassas, VA).

Techniques: Transduction, Plasmid Preparation, Generated, Injection, Derivative Assay, Isolation, Expressing

Journal: Cell Cycle

Article Title: Post-irradiation promotes susceptibility to reprogramming to pluripotent state in human fibroblasts

doi: 10.1080/15384101.2017.1371887

Figure Lengend Snippet: Characterization of induced pluripotent stem cells (iPSCs) generated from post-irradiated fibroblasts using four factors (OSKM) or three factors (OSK). (A) Morphology, immunostaining for pluripotent markers (Oct4, Sox2, and Nanog), and alkaline phosphatase (ALP) assay in 4F- or 3F-iPSCs (passage 7) derived from 5Gy-D14 cells. DAPI was used for counting nuclear staining. Scale bar = 100 μm. (B) Quantitative RT-PCR analysis of expression of endogenous pluripotent markers (Nanog, Oct4, and Sox2) in 4F- or 3F-iPSCs (5Gy-D14). mRNA expression levels of pluripotent markers was calculated relative to that of GAPDH and normalized to the parental cells as the control. The data are shown as means ± SDs from triplicate experiments (**P < 0.01, one-way ANOVA analysis with Scheffe pairwise post-hoc test) (C) DNA methylation analysis of several CpG sites in Oct4 and Nanog promoters, indicating demethylation of Oct4 and Nanog promoters in 4F- or 3F-iPSCs generated from 5Gy-D14 cells. Oct4 and Nanog promoters of parental cells were hyper-methylated. (D) Karyotyping of 4F- or 3F-iPSCs generated from 5Gy-D14 cells. NUFF: newborn human foreskin fibroblasts as donor cells. 4F-iPSC (5Gy-D14): iPSCs derived from fibroblasts at 14 d post-irradiation with 5 Gy using four factors (OSKM). 3F-iPSC (5Gy-D14): iPSCs derived from fibroblasts at 14 d post-irradiation with 5 Gy using three factors (OSK).

Article Snippet: Newborn human foreskin fibroblasts (passage 9) were purchased from Globalstem Inc. and maintained in DMEM containing 10% FBS.

Techniques: Generated, Irradiation, Immunostaining, ALP Assay, Derivative Assay, Staining, Quantitative RT-PCR, Expressing, DNA Methylation Assay, Methylation